Software Usage Function
HiFive hifive hic-normalize <SUBCOMMAND> [-h] [-m MINDIST] [-x MAXDIST] [-c CHROMS] [-o OUTPUT] [-q] [normalization options] project Find correction parameter values using one of the available algorithms.
computeMatrix computeMatrix reference-point -S <biwig file> -R <bed file> -a 3000 -b 3000 This tool prepares an intermediate file (a gzipped table of values) that contains scores associated with genomic regions. The regions can either be scaled to the same size (using the scale-regions mode) or you can choose the start, end, or center of each region as the focus point for the score calculations.
HiFive hifive fragments [-h] [-r RE] [-g GENOME] [-q] bed output Create a fragment file from a BED file containing targeted RE fragment data.
HiFive hifive fends [-h] (-F FEND | -B BED | -L LENGTH) [--binned] [-r RE] [-g GENOME] [-q] output Create a fend file from either a BED or HiCPipe-style fend file containing RE fragment data or create an arbitrarily-binned interval file from chromosome length file.
HiFive hifive hic-combine-replicates [-h] [-q] replicate1 replicate2 output Combine multiple HiC data files into a single file without needing to reload the data.
plotPCA plotPCA [-h] --corData FILE [--plotFile FILE] [--labels sample1 sample2 [sample1 sample2 ...]] [--plotTitle PLOTTITLE] [--plotFileFormat FILETYPE] [--plotHeight PLOTHEIGHT] [--plotWidth PLOTWIDTH] [--outFileNameData file.tab] [--ntop NTOP] [--PCs PCS PCS] [--log2] [--colors COLORS [COLORS ...]] [--markers MARKERS [MARKERS ...]] [--version] [--transpose | --rowCenter] This tool takes the default output file of multiBamSummary or multiBigwigSummary to perform a principal component analysis (PCA).
HiFive hifive hic-heatmap [-h] [-b BINSIZE] [-t] [-c CHROMS] [-d {raw,fend,distance,enrichment,expected}] [-F {hdf5,txt,npz}] [-y] [-x EXPBINSIZE] [-f MINOBS] [-a SEARCH] [-v] [-i IMAGE] [-p] [-l] [-n] [-k KEYWORDS] [-q] project output Using an already created HiC project, generate an HDF5-formatted heatmap file and optional image.
HiFive hifive hic-interval [-h] -c CHROM [-s START] [-e STOP] [-b BINSIZE] [-m MAXDIST] [-d {raw,fend,distance,enrichment,expected}] [-M] [-y] [-x EXPBINSIZE] [-f MINOBS] [-a SEARCH] [-v] [-i IMAGE] [-p] [-r] [-t] [-l] [-k KEYWORDS] [-q] project output Using an already created HiC project, generate a tabular genomic-interval or matrix file for a specified region and optional image.